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Oxidation of proteins: Basic principles and perspectives for blood proteomics
Author(s) -
Barelli Stefano,
Canellini Giorgia,
Thadikkaran Lynne,
Crettaz David,
Quadroni Manfredo,
Rossier Joël S.,
Tissot JeanDaniel,
Lion Niels
Publication year - 2008
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.200780009
Subject(s) - proteomics , oxidative stress , redox , chemistry , oxidative phosphorylation , oxidation reduction , biochemistry , reactive oxygen species , antioxidant , computational biology , biophysics , biology , organic chemistry , gene
Protein oxidation mechanisms result in a wide array of modifications, from backbone cleavage or protein crosslinking to more subtle modifications such as side chain oxidations. Protein oxidation occurs as part of normal regulatory processes, as a defence mechanism against oxidative stress, or as a deleterious processes when antioxidant defences are overcome. Because blood is continually exposed to reactive oxygen and nitrogen species, blood proteomics should inherently adopt redox proteomic strategies. In this review, we recall the biochemical basis of protein oxidation, review the proteomic methodologies applied to analyse redox modifications, and highlight some physiological and in vitro responses to oxidative stress of various blood components.