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Peptidomic analysis of rat urine using capillary electrophoresis coupled to mass spectrometry
Author(s) -
Frommberger Moritz,
Zürbig Petra,
Jantos Justyna,
Krahn Thomas,
Mischak Harald,
Pich Andreas,
Just Ingo,
SchmittKopplin Philippe,
Schiffer Eric
Publication year - 2007
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.200700195
Subject(s) - capillary electrophoresis , urine , chromatography , mass spectrometry , chemistry , albumin , urinary system , reproducibility , capillary electrophoresis–mass spectrometry , computational biology , biology , biochemistry , endocrinology , electrospray ionization
We have established and validated a protocol for the peptidomic analysis of rat urine using CE coupled to MS (CE‐MS). In the first experiments, the reproducibility of the CE‐MS set‐up and of the established preparation procedure were assessed. To establish a first rat urinary peptidome map, samples were also analyzed using CE‐FT‐ICR. The subsequent analysis of independent samples from two different strains (WISTAR and CD) indicated strain‐specific differences, which were validated in a blinded assessment. MS/MS revealed the presence of specific fragments from well‐known urinary rat peptides, such as collagens, alpha‐1‐antitrypsin, and serum albumin. The CE‐MS‐based peptidomics platform may provide novel insights into body fluids of animal models, such as rat or mice. Together with peptide identification, the technology appears to be an excellent, complimentary, and non‐invasive tool to analyze toxicological or other (patho)physiological effects of pharmaceutical compounds in animal models.