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Hemoglobin depletion from red blood cell cytosol reveals new proteins in 2‐D gel‐based proteomics study
Author(s) -
Bhattacharya Dipankar,
Mukhopadhyay Debashis,
Chakrabarti Abhijit
Publication year - 2007
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.200700178
Subject(s) - cytosol , hemoglobin , proteomics , red blood cell , biochemistry , chemistry , blood proteins , red cell , proteome , enzyme , medicine , gene
Proteomics studies to identify proteins in the erythrocyte cytosol have been largely affected by the huge abundance of hemoglobin (Hb), which masks the detection of other proteins in the 2‐D gel‐based separation. We have depleted Hb effectively from erythrocyte cytosol using cation exchange chromatography and have detected more than 600 protein spots in the Hb depleted hemolysate using 2‐DE. We have so far identified 59 proteins in the Hb‐depleted cytosol of normal erythrocytes, including 10 proteins not identified before.