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Quantitative proteomic approaches for biomarker discovery
Author(s) -
Hu Zhiyuan,
Hood Leroy,
Tian Qiang
Publication year - 2007
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.200700109
Subject(s) - biomarker discovery , biomarker , proteomics , computational biology , computer science , data science , quantitative proteomics , nanotechnology , bioinformatics , biology , materials science , biochemistry , gene
The rapid advances in proteomic technologies have made possible systematic analysis of hundreds to thousands of proteins in clinical samples with the promise of uncovering novel protein biomarkers for various disease conditions. We will discuss in this review article current MS and protein chip‐based quantitative proteomic approaches and their application in biomarker discovery. The emphasis will be placed on new quantification strategies employing stable isotopic labeling coupled with MS/MS, and antibody‐based protein chips and nanodevices. The strength and weakness of each technology are briefly highlighted.