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Two‐dimensional gel electrophoresis of apolipoprotein C‐III and other serum glycoproteins for the combined screening of human congenital disorders of O ‐ and N ‐glycosylation
Author(s) -
Bruneel Arnaud,
Robert Tiphaine,
Lefeber Dirk J.,
Benard Guillaume,
Loncle Emilie,
Djedour Amel,
Durand Geneviève,
Seta Nathalie
Publication year - 2007
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.200600777
Subject(s) - glycosylation , glycan , glycoprotein , transferrin , haptoglobin , apolipoprotein b , biochemistry , gel electrophoresis , sialic acid , population , biology , microbiology and biotechnology , chemistry , immunology , medicine , cholesterol , environmental health
Congenital disorders of glycosylation (CDG) are inherited diseases that can affect not only the N ‐glycan ( e.g. CDG type I and II) but also the O ‐glycan biosynthesis pathway. In the absence of specific clinical symptoms, there is a need for a reliable biological screening of these two groups of CDG. Using a few microlitres of human serum, 2‐DE and immunoblotting were applied to the separation and simultaneous detection of the isoforms of the O ‐glycosylated protein apolipoprotein C‐III (apoC‐III) and of four N ‐glycosylated proteins, namely alpha‐antitrypsin, alpha‐1 acid glycoprotein, haptoglobin and transferrin. For the study of O ‐glycosylation, this technique allowed the reliable separation of the three fractions of apoC‐III and the determination of normal percentage values in an adult population. Concerning N ‐glycosylation, the study of serum samples from patients with CDG type Ia revealed marked abnormalities systematically affecting the four 2‐DE separated N ‐linked glycoproteins. 2‐DE coupled to immunoblotting using a mixture of specific antibodies could be easily and reliably employed for the combined screening of both N ‐ and O ‐glycosylation disorders in humans.