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Identification of nasopharyngeal carcinoma antigens that induce humoral immune response by proteomic analysis
Author(s) -
Xiao ZhiQiang,
Chen Ying,
Yi Bin,
Li MaoYu,
Zhang PengFei,
Yi Hong,
Duan ChaoJun,
Li Cui,
Li JianLing,
Tang CenE,
Yang Fang,
Zhu GeQin,
Chen ZhuChu
Publication year - 2007
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.200600561
Subject(s) - nasopharyngeal carcinoma , autoantibody , western blot , cytokeratin , immune system , blot , immunohistochemistry , proteomics , antibody , antigen , immunology , pathology , immunoprecipitation , biology , medicine , cancer research , microbiology and biotechnology , gene , biochemistry , radiation therapy
Abstract A proteomics‐based approach has been used to identify proteins that commonly elicit a humoral immune response in nasopharyngeal carcinoma (NPC). Sera from 19 newly diagnosed NPC patients and 19 healthy individuals were analyzed for IgG autoantibodies against NPC proteins resolved by 2‐DE. Protein spots that exhibited selective reactivity with sera from NPC patients were identified by MS. Among nine identified proteins, cytokeratin 19 (CK19), Erb3 binding protein (EBP1), and Rho GDP dissociation inhibitor‐beta (Rho‐GDI‐2) induced autoantibodies in more than 36.8% of NPC patients but not in healthy individuals. Furthermore, Western blot analysis and immunohistochemical staining were performed to determine the expression and localization of CK19, EBP1, and Rho‐GDI‐2 in NPC and normal nasopharyngeal mucosal tissues. Up‐regulated CK19 and EBP1, but not Rho‐GDI‐2, were observed in NPC vs . normal tissue. Subcellular localization of the three proteins in NPC tissue was same as that in the normal tissue. Thus, overexpression of CK19 and EBP1 may be one of the mechanisms for their autoantibody development in NPC. To validate the findings of a proteomic analysis, occurrence of autoantibodies against these three proteins was detected by immunoprecipitation and Western blot analysis in additional 30 NPC patients, 23 other types of cancer patients and 20 healthy individuals. Results showed that frequency of autoantibodies against CK19, EBP1 and Rho‐GDI‐2 in NPC patients was significantly higher than that in other types of cancer patients and healthy individuals. We conclude that CK19, EBP1 and Rho‐GDI‐2 may have utility in NPC screening and diagnosis.