Premium
Effect of human umbilical cord‐derived mesenchymal stem cells on murine model of bronchiolitis obliterans like injury
Author(s) -
Lin Jilei,
Deng Huarong,
Zhang Yin,
Zou Lin,
Fu Zhou,
Dai Jihong
Publication year - 2021
Publication title -
pediatric pulmonology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.866
H-Index - 106
eISSN - 1099-0496
pISSN - 8755-6863
DOI - 10.1002/ppul.25128
Subject(s) - mesenchymal stem cell , medicine , bronchiolitis obliterans , umbilical cord , h&e stain , pathology , stem cell , andrology , lung , immunology , staining , lung transplantation , biology , genetics
Background Bronchiolitis obliterans is a fatal respiratory disease characterized by the obliteration of small airways. Mesenchymal stem cells (MSCs) is a promising candidate for cell‐based therapy. Objective To evaluate the therapeutic effect of human umbilical cord‐derived mesenchymal stem cells (HUC‐MSCs) on a murine model of bronchiolitis obliterans like injury (BOLI). Method The murine model of BOLI was established by administrating of diacetyl (DA) via intratracheal instillation. Treatment of HUC‐MSCs or HUC‐MSCs culture medium (HUC‐MSCs‐CM) was conducted in the BOLI model. Results The pathogenic manifestations, lung function, and the number of neutrophils were similar between the oropharyngeal inhalation DA group (OPI‐DA), intratracheal instillation group (ITI‐DA); however, less reduction of weight and higher survival rate were observed in ITI‐DA groups. Compared with the control groups, the trend of weight loss was significantly reduced ( p < .05), and the pulmonary function was significantly improved ( p < .05) in HUC‐MSCs and HUC‐MSCs‐CM groups. Masson staining and hematoxylin and eosin staining showed that the deposition of collagen around bronchioles and blood vessels is less and airway epithelial cells and basal cells in lung tissue repaired better in HUC‐MSCs and HUC‐MSCs‐CM groups compared with the control groups. Immunofluorescence shows the expression of E‐cadherin and cytokeratin 5 (CK‐5) were significantly higher in HUC‐MSCs and HUC‐MSCs‐CM groups compared with control groups, while HUC‐MSCs themselves did not express E‐cadherin or CK‐5. The DiI label showed HUC‐MSCs gradually reduced after 2 days in the bronchus and 4 days in bronchiole. Conclusion HUC‐MSCs could help to repair airway epithelial cells in a murine model of BOLI. It might be related to paracrine factors of HUC‐MSCs.