z-logo
Premium
Molecular and clinical characteristics of severe Mycoplasma pneumoniae pneumonia in children
Author(s) -
Yan Chao,
Xue Guanhua,
Zhao Hanqing,
Feng Yanling,
Li Shaoli,
Cui Jinghua,
Ni Shanshan,
Sun Hongmei
Publication year - 2019
Publication title -
pediatric pulmonology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.866
H-Index - 106
eISSN - 1099-0496
pISSN - 8755-6863
DOI - 10.1002/ppul.24327
Subject(s) - mycoplasma pneumoniae , medicine , multiple loci vntr analysis , coinfection , pleural effusion , genotype , pneumonia , gastroenterology , mycoplasma pneumonia , typing , virology , variable number tandem repeat , virus , microbiology and biotechnology , gene , biology , biochemistry
Abstract Objectives To analyze the molecular and the clinical characteristics of Mycoplasma pneumoniae (Mp) pneumonia (MPP) and to explore the related factors predicting severe MPP (SMPP). Methods A total of 423 pediatric cases of MPP were retrospectively analyzed, in 2013‐2017, in Beijing, China. Clinical information was collected from the medical records. Mp‐positive specimens were characterized using P1 typing and multiple locus variable‐number tandem repeat analysis (MLVA). The macrolide resistance‐associated mutations were also detected. Results The predominant genotype was P1‐1 (88.2%) and M4‐5‐7‐2 (87.5%), whereas percentages of type P1‐2 and M3‐5‐6‐2 increased across the 5‐year period. The mutation rate of genotype M4‐5‐7‐2 (365/370, 98.6%) was significantly higher than that of the genotype M3‐5‐6‐2 (15/48, 32.25%; P  = 0.000). Overall, 180 (42.6%) of the 423 Mp‐positive patients were coinfected with other pathogens. Respiratory syncytial virus coinfection (24/180, 13.3%) was more common in cases typed M3‐5‐6‐2 (4/23, 17.4%) than that of M4‐5‐7‐2 (20/155, 12.9%; P  = 0.038). Pleural effusion accounted for 52.6% (169/321) of the observed complications. In the mono‐infection cases, cases typed M3‐5‐6‐2 (56%, 14/25) were significantly ( P  = 0.020) associated with pleural effusion compared with those typed M4‐5‐7‐2 (32.6%, 70/215); 84% (21/25) of specimens typed M3‐5‐6‐2 were diagnosed as SMPP, whereas 63.7% (137/215) of specimens typed M4‐5‐7‐2 were diagnosed as SMPP ( P  = 0.043). Conclusions In our study, we proposed for the first time that the mono‐infection patients with Mp typed M3‐5‐6‐2 appear to have a higher risk for progressing to SMPP. MLVA typing can provide hints on the clinical characteristics of Mpp.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here