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Natural surfactant combined with superoxide dismutase and catalase decreases oxidative lung injury in the preterm lamb
Author(s) -
Dani Carlo,
Corsini Iuri,
Longini Mariangela,
Burchielli Silvia,
Dichiara Giulia,
Cantile Carlo,
Buonocore Giuseppe
Publication year - 2014
Publication title -
pediatric pulmonology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.866
H-Index - 106
eISSN - 1099-0496
pISSN - 8755-6863
DOI - 10.1002/ppul.22955
Subject(s) - superoxide dismutase , pulmonary surfactant , catalase , oxidative stress , respiratory distress , lung , medicine , chemistry , biochemistry , anesthesia
Summary We performed a randomized study in preterm lambs to assess the hypothesis that treatment with a natural surfactant combined with superoxide dismutase (SOD) and catalase (CAT) might decrease pulmonary oxidative stress in an animal model of respiratory distress syndrome (RDS). Animals received 200 mg/kg of porcine natural surfactant or 200 mg/kg of natural surfactant combined with 2 mg/ml of SOD and 3,000 U/ml of CAT. Lung tissue oxidation was studied by measuring total hydroperoxide (TH), advanced oxidation protein products (AOPP), and non‐protein bound iron (NPBI) in bronchial aspirate samples. In addition, the animal's lung mechanics were evaluated. TH, AOPP, and NPBI were lower in the groups treated with surfactant plus SOD and CAT than in the surfactant group, while lung mechanics did not vary. We concluded that natural surfactant combined with SOD and CAT is effective in reducing the oxidative lung stress in an animal model of RDS. Pediatr Pulmonol. 2014; 49:898–904. © 2013 Wiley Periodicals, Inc.