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Reversed changes of lipoxin A 4 and leukotrienes in children with asthma in different severity degree
Author(s) -
Wu ShengHua,
Yin PeiLing,
Zhang YongMei,
Tao HuiXian
Publication year - 2010
Publication title -
pediatric pulmonology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.866
H-Index - 106
eISSN - 1099-0496
pISSN - 8755-6863
DOI - 10.1002/ppul.21186
Subject(s) - medicine , asthma , leukotriene , immunology , lipoxin , immunoglobulin e , whole blood , respiratory disease , gastroenterology , endocrinology , inflammation , lung , antibody
Objectives To investigate the expressions of 15‐lipoxygenase (15‐LO) and 5‐lipoxygenase (5‐LO) in leukocytes and the changes of blood lipoxin A 4 (LXA 4 ), leukotriene (LT)B 4 and LTC 4 in children with asthma, and to explore the relationship between the blood eicosanoids and one of serum high‐sensitivity C‐reactive protein (hsCRP), interleukin (IL)‐5, IL‐8 and IL‐13 and IgE in children with asthma. Study Design One hundred six asthmatic children were divided into three groups, that is, mild persistent asthmatic group, moderate persistent asthmatic group and severe persistent asthmatic group. Forty healthy children were served as controls. Methodology The expressions of 15‐LO and 5‐LO mRNA in leukocytes were assessed by reverse transcription‐polymerase chain reaction, and the blood LXA 4 , LTB 4 , LTC 4 , IL‐5, IL‐8, and IL‐13 were determined with enzyme‐linked immunosorbent assay. Serum hsCRP was determined with latex‐enhanced immuno‐turbidimetry kits. Results The leukocytic 15‐LO expression and blood LXA 4 were gradually decreased, and the leukocytic 5‐LO expression, blood LTB 4 , LTC 4 , IL‐5, IL‐8, IL‐13, and hsCRP were gradually increased in children with asthma from mild degree to moderate and severe degree. There were positive correlations between blood LXA 4 and one of the peak expiratory flow (PEF) and forced expiratory volume in 1 sec (FEV 1 ) percent‐predicted values, and negative correlations between blood LTC 4 and one of the PEF and FEV 1 percent‐predicted values in children with asthma. There were negative correlations between blood LXA 4 and one of the IL‐5, IL‐8, IL‐13, and hsCRP levels, and positive correlations between one of blood LTB 4 , LTC 4 and one of the IL‐5, IL‐8, IL‐13 and hsCRP levels in children with asthma. Conclusions The reversed changes between 15‐LO, its product LXA 4 and 5‐LO, its products LTB 4 and LTC 4 in children with asthma from mild, moderate to severe degree were found, suggesting that insufficient generation of LXA 4 and overproduction of LTs may be the reason for the asthmatic children whose illness become more serious. Pediatr Pulmonol. 2010; 45:333–340. © 2010 Wiley‐Liss, Inc.