Premium
Association of haplotypes of β 2 ‐adrenoceptor polymorphisms with lung function and airway responsiveness in a pediatric cohort
Author(s) -
Zhang G.,
Hayden C.M.,
Khoo S.K.,
Laing I.A.,
Turner S.,
Landau L.,
Goldblatt J.,
Le Souëf P.N.
Publication year - 2006
Publication title -
pediatric pulmonology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.866
H-Index - 106
eISSN - 1099-0496
pISSN - 8755-6863
DOI - 10.1002/ppul.20529
Subject(s) - haplotype , medicine , single nucleotide polymorphism , cohort , asthma , prospective cohort study , lung function , β2 adrenergic receptor , lung , gastroenterology , genotype , pediatrics , genetics , receptor , biology , gene , agonist
We evaluated the influence of haplotypes of β 2 ‐adrenergic receptor (ADRB2) polymorphisms on lung function and airway responsiveness (AR) in a pediatric cohort recruited before birth and followed up to 11 years of age. The subjects (180) were the participants in a prospective study of lung function and AR. They have been assessed five times (at 1 month, 6 months, 12 months, 6 and 11 years of age) for lung function and AR. The two ADRB2 single nucleotide polymorphisms (SNPs): Arg16Gly and Gln27Glu were genotyped by PCR‐RLFP and their haplotypes inferred using the program PHASE. An association between the haplotype arg16gln27 and the prevalence of positive AR was found at age 6 years ( P = 0.009). The gly16gln27 haplotype was associated with higher FEV1 ( P = 0.015) at age 6 and both higher FEV1 and FVC ( P = 0.018 and P = 0.001, respectively) at age 11. In contrast, arg16gln27 was associated with both lower FEV1 and FVC ( P = 0.028 and P = 0.011, respectively) at age 11. Children with the gly16gln27 haplotype were less likely to have asthma‐ever or doctor‐diagnosed asthma at age 11 (OR: 0.38; P = 0.019 and OR: 0.31; P = 0.041, respectively). In conclusion, haplotypes of β 2 ‐adrenoceptor polymorphisms are associated with lung function, AR, and asthma susceptibility in childhood. Pediatr Pulmonol. 2006; 41: 1233–1241. © 2006 Wiley‐Liss, Inc.