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Brain cell membrane modification following hypercapnia and recovery in newborn piglets
Author(s) -
Schneiderman Roy,
Kubin Joanna,
Mishra Om Prakash,
DelivoriaPapadopoulos Maria
Publication year - 1994
Publication title -
pediatric pulmonology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.866
H-Index - 106
eISSN - 1099-0496
pISSN - 8755-6863
DOI - 10.1002/ppul.1950180205
Subject(s) - medicine , hypercapnia , brain cell , intensive care medicine , anesthesia , neuroscience , acidosis , biology
The effect of hypercapnia on brain cell membrane structure and function was studied in anesthetized newborn piglets. Lipid peroxidation products (conjugated dienes and fluorescent compounds), Na + ,K + ‐ATPase activity and enzyme affinity to ATP (substrate), K + and Na + ions (activators), and strophanthidin (inhibitor) were measured in three groups of animals: controls, those exposed to 90 minutes of P aco 2 > 80 mmHg (hypercapnia) and those exposed the same way, following restoration of normal P aco 2 , (recovery). Enzyme activity was unchanged by hypercapnia, but enzyme affinity was altered as indicated by an increase in ATP affinity. Affinities to Na + , K + , and strophanthidin were unchanged. Restoration of normal P aco 2 resulted in an increase in conjugated dienes. The data demonstrate that hypercapnia followed by restoration of normal P aco 2 in healthy term newborn piglets is associated with mild modification of brain cell membrane Na + , K + ‐ATPase, possibly due to lipid peroxidation. Pediatr Pulmonal. 1994;18:81–88. © 1994 Wiley‐Liss, Inc.