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Prevention of chronic pulmonary oxygen toxicity in young rats with liposome‐encapsulated catalase administered intratracheally
Author(s) -
Thibeault D. W.,
Rezaiekhaligh M.,
Mabry S.,
Beringer T.
Publication year - 1991
Publication title -
pediatric pulmonology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.866
H-Index - 106
eISSN - 1099-0496
pISSN - 8755-6863
DOI - 10.1002/ppul.1950110408
Subject(s) - oxygen toxicity , catalase , medicine , parenchyma , superoxide dismutase , toxicity , pulmonary toxicity , lung , room air distribution , glutathione peroxidase , pathology , right ventricular hypertrophy , respiratory system , endocrinology , pulmonary hypertension , oxidative stress , physics , thermodynamics
The lungs and hearts of young rats exposed to 100% oxygen (O 2 ) for 8 days (27 to 35 days of age) were studied following recovery in room air at 60 days of age using morphometric, biochemical, and physiological techniques. In an attempt to prevent chronic oxygen toxicity 153 rats had transtracheal catheters surgically implanted and were treated during the O 2 exposure with daily intratracheal injections of liposome‐encapsulated superoxide dismutase (SOD) and/or catalase (CAT). Oxygen exposure in this model results in chronic cardiopulmonary alterations which include pulmonary hypertension, right ventricular hypertrophy, and a decrease in number of pulmonary arterioles 25 to 50 μm in diameter with increased muscularization of their walls. The volume densities of the parenchyma, parenchymal air space, and the alveolar space are increased, while that of the combined alveolar ductal and respiratory bronchiolar space is decreased. Daily intratracheal administration of liposome‐encapsulated CAT (160 U) during the O 2 exposure prevented these chronic changes. Liposome‐encapsulated SOD (110 U) or SOD (50 U) + CAT (70 U) did not appear to have a preventive effect. During the first 3 to 5 days following oxygen exposure the lung tissue enzymes SOD, CAT, and glutathione peroxidase markedly increased. We conclude that in the young rat animal model liposomeencapsulated CAT (160 U) given intratracheally during the period of O 2 exposure is safe and will prevent the chronic vascular and parenchymal damage due to oxygen toxicity.