Premium
Genotypic characterization of Pseudomonas aeruginosa strains recovered from patients with cystic fibrosis after initial and subsequent colonization
Author(s) -
Munck Anne,
Bonacorsi Stéphane,
MarianiKurkdjian Patricia,
Lebourgeois Muriel,
Gérardin Michèle,
Brahimi Naïma,
Navarro Jean,
Bingen Edouard
Publication year - 2001
Publication title -
pediatric pulmonology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.866
H-Index - 106
eISSN - 1099-0496
pISSN - 8755-6863
DOI - 10.1002/ppul.1121
Subject(s) - colonization , cystic fibrosis , pseudomonas aeruginosa , tobramycin , colistin , genotype , medicine , colonisation , antibiotics , microbiology and biotechnology , persistence (discontinuity) , respiratory disease , chronic infection , biology , bacteria , immunology , lung , gentamicin , gene , biochemistry , genetics , geotechnical engineering , immune system , engineering
Chronic infection by Pseudomonas aeruginosa (PA) in patients with cystic fibrosis (CF) is preceded by a period of colonization and acute infection. Early aggressive antibiotic treatment of initial colonisation may prevent or at least delay chronic pulmonary infection. We initiated treatment with a combination of IV β‐lactam tobramycin, followed by nebulized colistin when PA was first isolated from patients with CF. Subsequent serial PA isolates obtained from these colonized CF patients were characterized by means of molecular methods to determine whether they were genetically related to the initial strain. Initial colonization was eradicated in all 19 patients. All patients reacquired PA within 3–25 months during the 3 years of follow‐up. Fourteen patients acquired a new PA strain with a distinct genotypic profile, suggesting a new source of contamination. Five patients had two PA isolates with identical genotypes, suggesting either previous undetected respiratory tract colonization or a persistent environmental source of contamination. Pediatr Pulmonol. 2001; 32:288–292. © 2001 Wiley‐Liss, Inc.