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Safety and use of sputum induction in children with cystic fibrosis
Author(s) -
Suri Ranjan,
Marshall Lindsay J.,
Wallis Colin,
Metcalfe Christopher,
Shute Janis K.,
Bush Andrew
Publication year - 2003
Publication title -
pediatric pulmonology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.866
H-Index - 106
eISSN - 1099-0496
pISSN - 8755-6863
DOI - 10.1002/ppul.10226
Subject(s) - hypertonic saline , sputum , medicine , cystic fibrosis , pseudomonas aeruginosa , staphylococcus aureus , bronchoconstriction , stenotrophomonas maltophilia , inhalation , eosinophil cationic protein , neutrophil elastase , microbiology and biotechnology , proinflammatory cytokine , immunology , gastroenterology , eosinophil , anesthesia , asthma , pathology , inflammation , bacteria , biology , tuberculosis , genetics
We assessed the safety and use of induced sputum (IS) in children with cystic fibrosis (CF). Forty‐eight children (19 males) with CF, mean age 12.6 (range, 7.3–17.0) years and median forced expired volume in 1 sec (FEV 1 ) 48% (range, 14–77%) predicted were recruited. Patients spontaneously expectorated sputum and then performed sputum induction by inhalation of nebulized 7% hypertonic saline. Samples were sent for bacteriological culture, and for measurement of the following inflammatory mediators: interleukin‐8, myeloperoxidase, eosinophil cationic protein, and neutrophil elastase activity. FEV 1 was performed before and after inhalation of hypertonic saline. There was no increase in mediator levels in IS compared to expectorated sputum (ES) samples. Only 3 patients demonstrated significant bronchoconstriction following inhalation of hypertonic saline, by the method used. From the ES samples, Pseudomonas aeruginosa was isolated in 13 patients, Staphylococcus aureus in 7 patients, Stenotrophomonas maltophilia in 1 patient, and both Pseudomonas aeruginosa and Staphylococcus aureus in 5 patients. All these organisms were found in the IS samples. However, in 2 patients whose ES grew no organisms, one patient's IS grew Pseudomonas aeruginosa , and the other patient's IS grew Staphylococcus aureus . In our study, sputum induction was safe, with no proinflammatory effect. Pediatr Pulmonol. 2003; 35:309–313. © 2003 Wiley‐Liss, Inc.