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Selective Targeting of Lymphoma Cells by Monoclonal Antibody Grafted onto Zwitterionic‐Functionalized Nanoparticles
Author(s) -
Oliveira Jessica Fernanda Affonso,
da Silva Raquel Frenedoso,
Ribeiro Iris Renata Sousa,
Saito Ângela,
MeloHanchuk Talita Diniz,
Dias Marília Meira,
Kobarg Jörg,
Cardoso Mateus Borba
Publication year - 2020
Publication title -
particle and particle systems characterization
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 56
eISSN - 1521-4117
pISSN - 0934-0866
DOI - 10.1002/ppsc.201900446
Subject(s) - internalization , monoclonal antibody , nanomedicine , chemistry , cytotoxicity , drug delivery , cancer cell , surface modification , nanoparticle , nanotechnology , biophysics , antibody , cell , biochemistry , cancer , materials science , in vitro , biology , immunology , genetics , organic chemistry
Nanomedicine is considered a promising alternative to improve cancer diagnosis and treatment. Particularly, the use of nanoparticles (NPs) has enabled the encapsulation of highly toxic anticancer drugs, facilitated ultimate targeting, and allowed tailoring of drug delivery. However, when in biological fluids, these NPs are coated by proteins which hide the targeting moieties and suppress the engineered biological outcome. Herein, how the Ki‐1 monoclonal antibody (mAb) can preserve its targetability through grafting on the surface of zwitterionic‐functionalized nanoparticles, is unveiled. Zwitterions, known for their stealth ability, are used to minimize unspecific NPs protein adsorption and consequently maintain mAb functionality. In this work, Ki‐1 mAb is used as it recognizes TNFRSF8 (CD30 + ) transmembrane protein overexpressed on CD30 + lymphoma cells such as L540 cells. While nonfunctionalized NPs show negligible toxic effects toward L540 cells, the Ki‐1‐functionalized structure demonstrates cytotoxicity, since they undergo cellular uptake, suggesting a receptor‐mediated internalization. This dual‐functionalization strategy provides a promising multifunctional nanoplatform toward future personalized medicine applications, minimizing unspecific protein adsorption on NPs and ensuring selective cancer cell targeting.