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Core–Shell Poly (Ionic Liquid)@Mesoporous Silica Chemiluminescent Nanoprobes for Sensitive Intracellular Hydrogen Peroxide Imaging
Author(s) -
Zhou Kai,
Zhang Feng,
Xu Jing,
He Hui,
Wei Weili,
Xia Zhining
Publication year - 2018
Publication title -
particle and particle systems characterization
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 56
eISSN - 1521-4117
pISSN - 0934-0866
DOI - 10.1002/ppsc.201700329
Subject(s) - nanoprobe , intracellular , hydrogen peroxide , chemistry , fluorescence , chemiluminescence , biophysics , ionic liquid , mesoporous silica , in vivo , ionic bonding , mesoporous material , nanoparticle , nanotechnology , materials science , chromatography , biochemistry , catalysis , organic chemistry , ion , physics , microbiology and biotechnology , quantum mechanics , biology
Despite significant developments in spatial distribution imaging of H 2 O 2 as one the most important nonradical reactive oxygen species, novel background‐free, highly sensitive, and selective probes that allow intracellular sensing are still imperative. This is mainly because the fluorescent probes usually suffer some drawbacks such as, fluorescence bleaching and requirement of bulky light sources. In this study, the rational design and fabrication of a nonenzymatic nanoprobe (c‐PIL@mSiO 2 ) with dramatically improved sensitivity for chemiluminescent (CL) imaging of intracellular and in vivo H 2 O 2 at nano molar level is presented. The limit of detection is lower than the endogenous H 2 O 2 concentration, and is significantly better than that of some recently reported fluorescent and CL probes. Structurally, the nanoprobe is composed of a unique amphiphilic poly(ionic liquid) core for preserving H 2 O 2 responsive reagents, and a mesoporous silica shell acts as an “exoskeleton” to provide hydrophilic nature. The multiple alternating hydrophobic and hydrophilic nanodomains of the poly(ionic liquid) core increase mass transfer dynamics, which increase the sensitivity of H 2 O 2 imaging. RAW264.7 macrophages and mice models of inflammations experiment show that the c‐PIL@mSiO 2 is capable of imaging H 2 O 2 intracellular and in vivo. This probe for the first time achieves CL detection of endogenous intracellular H 2 O 2 without disruption of cells.