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Polyacrylamide Nanoparticles with Visible and Near‐Infrared Autofluorescence
Author(s) -
Xie Hongmei,
Zhang Ling,
Wu Lin,
Wang Jinke
Publication year - 2017
Publication title -
particle and particle systems characterization
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 56
eISSN - 1521-4117
pISSN - 0934-0866
DOI - 10.1002/ppsc.201700222
Subject(s) - autofluorescence , biocompatibility , nanoparticle , zeta potential , nanomaterials , fluorescence , photosensitizer , nanotechnology , chemistry , photodynamic therapy , polyacrylamide , biophysics , materials science , photochemistry , polymer chemistry , optics , physics , organic chemistry , biology
Nowadays, self‐fluorescent materials such as quantum dots are widely studied and applied in biomedical field. However, the biggest obstacle is biocompatibility. Here, a novel autofluorescent nanoparticle is constructed by crosslinking polyacrylamide nanoparticles (PAANPs) that contain ε‐poly‐ l ‐lysine with glutaraldehyde (named fPAANPs). The nanoparticle has a mean size of about 16 nm, a zeta potential of about +16 mV, and strong visible and near‐infrared autofluorescence. The nanoparticle can be efficiently internalized into cells with high biocompatibility, the LC50 of which in RAW264.7, HepG2, and Hepa1‐6 cells is 6, 9, and 7.5 mg mL −1 , respectively. The nanoparticle shows no visible impact on the mice vitality even at a high intravenously administered dose (126 mg kg −1 ). The autofluorescence of fPAANPs shows high stability, persistence, allowing long‐term dynamic imaging for 25 d in subcutaneous injections and 18 d in xenograft tumors in mice. The nanoparticle thus provides a self‐traceable nanomaterial that can be exploited as drug carrier and potential photodynamic therapy photosensitizer.