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Metal–Polymer Hybrid Nanoparticles for Correlative High‐Resolution Light and Electron Microscopy
Author(s) -
Reifarth Martin,
Preußger Elisabeth,
Schubert Ulrich S.,
Heintzmann Rainer,
Hoeppener Stephanie
Publication year - 2017
Publication title -
particle and particle systems characterization
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 56
eISSN - 1521-4117
pISSN - 0934-0866
DOI - 10.1002/ppsc.201700180
Subject(s) - correlative , electron microscope , microscopy , nanoparticle , transmission electron microscopy , nanotechnology , fluorescence microscope , biophysics , chemistry , fluorescence , materials science , chemical engineering , biology , optics , philosophy , linguistics , physics , engineering
The combination of fluorescence microscopy and electron microscopy promises a deeper insight into the ultrastructural features of cell organelles, e.g., after drug administration. Both methods complement each other and provide, as a correlative approach, a keen insight into the fate of nanoparticles within the cell. Moreover, it represents a promising tool to determine alterations of the cellular environment as a response to particle uptake. However, the availability of suitable correlative markers is mandatory for such correlative approaches. In this contribution, the utilization of poly(ethylene imine) based metal–polymer hybrid particles labeled with small gold nanoparticles and Rhodamine B facilitating the observation of the particles by means of fluorescence as well as by transmission electron microscopy is suggested. Correlative light and electron microscopy is used to study uptake and intracellular fusion processes of endosomal/lysosomal structures.