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Two‐Photon Imaging of a Cellular Line Using Organic Fluorescent Nanoparticles Synthesized by Laser Ablation
Author(s) -
AparicioIxta Laura,
AlbaRosales J. E.,
RamosOrtiz Gabriel,
Rodriguez Mario,
PichardoMolina Juan L.,
GutierrezJuarez Gerardo,
SabaneroLopez Myrna,
Villavicencio Liss Flores,
Santillan Rosa,
TellezLopez Víctor M.,
MartinezFong Daniel
Publication year - 2016
Publication title -
particle and particle systems characterization
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 56
eISSN - 1521-4117
pISSN - 0934-0866
DOI - 10.1002/ppsc.201500199
Subject(s) - nanoparticle , fluorescence , aqueous solution , absorption (acoustics) , materials science , laser ablation , two photon excitation microscopy , fluorescence microscope , microscopy , photochemistry , absorption spectroscopy , laser , conjugated system , chemistry , analytical chemistry (journal) , nanotechnology , polymer , optics , organic chemistry , physics , composite material
A comparative study of the optical properties of organic fluorescent nanoparticles fabricated by laser ablation (NPs‐LA), reprecipitation (NPs‐RP), and microemulsion (NPs‐ME) methods is presented. These nanoparticles contain a fluorene‐based p‐conjugated molecule (BT2). Distinctive electronic transitions are observed in samples due to the specific way in which the molecule BT2 is assembled in each type of nanoparticles; for instance, transitions involved in absorption and emission spectra of NPs‐LA result in blueshifting with respect to the molecular solution of BT2, whereas redshifting is observed in NPs‐RP and NPs‐ME. Further, the results show that under infrared excitation, the aqueous suspensions of NPs‐LA exhibit the highest fluorescence induced by two‐photon absorption (≈790 GM at 740 nm), as well as the best photostability, compared with aqueous suspensions of NPs‐RP and NPs‐ME. The nanoparticles synthetized by the three aforementioned methods are employed as exogenous agents for the visualization of human cervical cancer cell line (HeLa) using confocal and two‐photon microscopy. Under similar experimental conditions, it is found that microscopy images of the best quality are obtained with NPs‐LA. These results show that laser ablation is a suitable technique for the fabrication of organic fluorescent nanoparticles used as contrast agents for in vitro fluorescence microscopy.