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Nanoparticle Aggregate‐Based Fluorescence Enhancement for Highly Sensitive and Reproducible Detection of DNA
Author(s) -
Annink Carla,
Gill Ron
Publication year - 2014
Publication title -
particle and particle systems characterization
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 56
eISSN - 1521-4117
pISSN - 0934-0866
DOI - 10.1002/ppsc.201400061
Subject(s) - fluorescence , dna , nanoparticle , chemistry , detection limit , analytical chemistry (journal) , materials science , chromatography , nanotechnology , biochemistry , optics , physics
Sensitive detection of DNA at the sub picomolar range is demonstrated using a magnetic bead sandwich hybridization assay coupled with surface‐enhanced fluorescence (SEF)‐based amplification. Unlike enzymatic amplification, the SEF amplification step does not add any additional background to the measurement and does not cause a significant increase in the coefficient of variance (CV).