Premium
Evaluation of Rod‐Shaped Nanoparticles as Carriers for Gene Delivery
Author(s) -
Fan MinMin,
Zhang WanZhen,
Cheng Cong,
Liu Yan,
Li BangJing,
Sun Xun,
Zhang Sheng
Publication year - 2014
Publication title -
particle and particle systems characterization
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 56
eISSN - 1521-4117
pISSN - 0934-0866
DOI - 10.1002/ppsc.201300383
Subject(s) - polyethylenimine , gene delivery , ethylene glycol , cytotoxicity , nanoparticle , dna , chemistry , nanotechnology , biophysics , fabrication , gene , materials science , genetic enhancement , transfection , biochemistry , biology , organic chemistry , medicine , alternative medicine , pathology , in vitro
Fabrication of nonspherical particles for gene delivery remains a major challenge. In this study, novel rod‐like nanoparticles are prepared for efficient gene delivery by self‐assembly of α‐cyclodextrin (α‐CD) and polyethylenimine‐methoxy poly(ethylene glycol) (PEI‐mPEG). The study reveals that the rod‐like PEI‐mPEG/α‐CD particles can bind DNA effectively and the resulting PEI‐mPEG/α‐CD/DNA complexes show over four times higher gene delivery capability than their spherical counterparts and PEI(25K) due to more efficient cellular uptake. Furthermore, the cytotoxicity of rod‐like PEI‐mPEG/α‐CD is about five times lower than that of the nanospheres, and 50 times lower than that of DNA/PEI(25K). These results indicate that shape is an important parameter for the design of gene delivery vectors.