Premium
Identification of key neutral species in atmospheric‐pressure plasma for promoting proliferation of fibroblast cells
Author(s) -
Hori Yuki,
Iwata Naoyuki,
Gamaleev Vladislav,
Oh JunSeok,
Murata Tomiyasu,
Hori Masaru,
Ito Masafumi
Publication year - 2021
Publication title -
plasma processes and polymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 74
eISSN - 1612-8869
pISSN - 1612-8850
DOI - 10.1002/ppap.202000225
Subject(s) - radical , atmospheric pressure , atmospheric pressure plasma , plasma , fibroblast , chemistry , cell growth , promotion (chess) , generator (circuit theory) , analytical chemistry (journal) , biophysics , materials science , biochemistry , chromatography , biology , in vitro , physics , political science , law , quantum mechanics , politics , meteorology , power (physics)
To identify the key neutral species from atmospheric‐pressure plasma for promoting the proliferation of fibroblast cells, an atmospheric‐pressure radical generator was employed. A cell viability assay showed that the proliferation of fibroblasts was significantly promoted through radical treatment, resulting in the highest promotion ratio of 24%. The densities of the neutral radicals supplied by the radical generator were measured via mass spectrometry at atmospheric pressure. To determine an effective factor for the promotion, the dose dependencies of these radicals on the promotion ratio were investigated. The promotion ratio exhibited a strong correlation only with the NO• dose. These results confirmed that NO• is a direct trigger of plasma‐enhanced cell proliferation.