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Controlled co‐immobilization of biomolecules on quinone‐bearing plasma polymer films for multifunctional biomaterial surfaces
Author(s) -
Czuba Urszula,
MorenoCouranjou Maryline,
Collard Delphine,
De PauwGillet MarieClaire,
Quintana Robert,
Lassaux Patricia,
Detrembleur Christophe,
Choquet Patrick
Publication year - 2020
Publication title -
plasma processes and polymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 74
eISSN - 1612-8869
pISSN - 1612-8850
DOI - 10.1002/ppap.202000090
Subject(s) - biomolecule , quartz crystal microbalance , chemical engineering , polymer , x ray photoelectron spectroscopy , chemistry , materials science , polymerization , nanotechnology , organic chemistry , adsorption , engineering
In this study, an efficient methodology, allowing the controlled co‐immobilization of two complementary biomolecules, is reported for the production of multifunctional antibacterial surfaces. To promote long‐lasting covalent immobilization, metallic surfaces are first coated with a quinone‐bearing poly(methacrylate)‐based thin film by combining an atmospheric pressure liquid‐assisted plasma polymerization and a controlled sodium periodate‐induced catechol oxidation steps. The influence of the oxidation step on the film morphology and chemistry is investigated using an analytical multitool approach involving atomic force microscopy, ultraviolet, infrared, and X‐ray photoelectron spectroscopy techniques. Quartz crystal microbalance with dissipation monitoring (QCM‐D) analyses allow the rapid determination of the optimal biomolecule immobilization conditions in terms of kinetics of grafting and biomolecule solution concentrations. In vitro functional assays combined with QCM‐D analyses demonstrate promising, dual biologically active coated surfaces.