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Atmospheric pressure plasma treatments protect neural cells from ischemic stroke‐relevant injuries by targeting mitochondria
Author(s) -
Yan Xu,
Zhang Chenyang,
Ouyang Jiting,
Shi Zhongfang,
Chen Ye,
Han Ruoyu,
Zhang Wei,
Yuan Fang,
Ostrikov Kostya Ken
Publication year - 2020
Publication title -
plasma processes and polymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 74
eISSN - 1612-8869
pISSN - 1612-8850
DOI - 10.1002/ppap.202000063
Subject(s) - apoptosis , mitochondrion , reactive oxygen species , cytochrome c , oxidative stress , intracellular , atmospheric pressure plasma , microbiology and biotechnology , cytoplasm , cell , chemistry , programmed cell death , oxidative phosphorylation , biophysics , biochemistry , biology , plasma , physics , quantum mechanics
Most studies regarding plasma biomedicine applications mainly focus on the oxidative and/or nitrative stress on bacteria, cancer cells, and other treatment objects. In this study, we evaluate the protective effect of appropriate atmospheric pressure plasma jet (APPJ) treatments on oxygen and glucose deprivation (OGD)‐induced neural cell apoptosis, which is a major pathological process during ischemic stroke, based on the physiological functions of NO. Results show that APPJ treatment reduces the OGD‐induced apoptosis by weakening typical OGD injury consequences including loss of mitochondrial membrane potential, the release of cytochrome c from the mitochondria into the cytoplasm, lower antiapoptotic Bcl‐2 expression, and upregulating the proapoptotic protein Bax. Furthermore, APPJ increased intracellular NO production, which is closely related to the cytoprotective effect of APPJ.