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Rapid inactivation of airborne porcine reproductive and respiratory syndrome virus using an atmospheric pressure air plasma
Author(s) -
Nayak Gaurav,
Andrews Austin J.,
Marabella Ian,
Aboubakr Hamada A.,
Goyal Sagar M.,
Olson Bernard A.,
Torremorell Montserrat,
Bruggeman Peter J.
Publication year - 2020
Publication title -
plasma processes and polymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 74
eISSN - 1612-8869
pISSN - 1612-8850
DOI - 10.1002/ppap.201900269
Subject(s) - porcine reproductive and respiratory syndrome virus , infectivity , virus , dielectric barrier discharge , virology , chemistry , titer , airborne transmission , reverse transcription polymerase chain reaction , atmospheric pressure , biology , covid-19 , biochemistry , medicine , infectious disease (medical specialty) , gene , oceanography , disease , electrode , pathology , messenger rna , geology
The transmission of airborne diseases in animals poses great risks to animal safety with potential significant economic losses. In this study, we report on the use of a dielectric barrier discharge (DBD) for in‐flight inactivation of an airborne aerosolized porcine reproductive and respiratory syndrome virus. The infectivity of the sampled virus downstream compared to upstream of the DBD reactor as determined by the TCID 50 method showed a ∼3.5 log 10 reduction in the virus titer. Independent testing of the viral genome by the reverse‐transcription quantitative real‐time polymerase chain reaction method confirmed the inactivation with minimal filtering effects. Both short‐lived species such as OH• and O 2 ( a 1 Δ g ) and peroxynitrous acid (ONOOH) chemistry at low pH in the virus‐laden droplets are suggested to be responsible for the observed inactivation.