Premium
Intracellular responses to reactive oxygen and nitrogen species, and lipid peroxidation in apoptotic cells cultivated in plasma‐activated medium
Author(s) -
Furuta Ryo,
Kurake Naoyuki,
Ishikawa Kenji,
Takeda Keigo,
Hashizume Hiroshi,
Tanaka Hiromasa,
Kondo Hiroki,
Sekine Makoto,
Hori Masaru
Publication year - 2017
Publication title -
plasma processes and polymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 74
eISSN - 1612-8869
pISSN - 1612-8850
DOI - 10.1002/ppap.201700123
Subject(s) - intracellular , hela , reactive oxygen species , lipid peroxidation , apoptosis , extracellular , chemistry , microbiology and biotechnology , biophysics , membrane , biochemistry , oxidative stress , cell , biology
A mechanism of the cytotoxicity of plasma‐activated medium (PAM) is revealed by examining the intracellular effects of reactive oxygen nitrogen species (RONS) and lipid oxidation. PAM is cell culture medium activated by irradiation using non‐equilibrium atmospheric ‐ pressure plasma using pure Ar gas in ambient air. Extracellular RONS in PAM induced the apoptotic death of HeLa cells. Temporal changes in intracellular RONS, such as ONOO − , NO, andO 2 − , were analyzed. Intracellular RONS generation in HeLa cells following incubation in PAM triggered activation of the caspase cascade pathway and lipid peroxidation of intracellular membranes to induce apoptosis.