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Direct plasma stimuli including electrostimulation and OH radical induce transient increase in intracellular Ca 2+ and uptake of a middle‐size membrane‐impermeable molecule
Author(s) -
Sasaki Shota,
Hokari Yutaro,
Kumada Akiko,
Kanzaki Makoto,
Kaneko Toshiro
Publication year - 2018
Publication title -
plasma processes and polymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 74
eISSN - 1612-8869
pISSN - 1612-8850
DOI - 10.1002/ppap.201700077
Subject(s) - biophysics , intracellular , chemistry , mannitol , plasma , membrane , irradiation , molecule , hydroxyl radical , cell membrane , radical , biochemistry , organic chemistry , physics , quantum mechanics , nuclear physics , biology
Despite the promising potential of medical treatments based on non‐equilibrium atmospheric‐pressure plasma, most of the underlying mechanisms remain unknown. Here we report that direct plasma irradiation (DPI) can induce transient increase in intracellular calcium (Ca 2+ ) concentrations, thereby playing key roles in many cellular processes as well as the uptake of middle‐size membrane‐impermeable molecules, which did not occur with indirect plasma irradiation (IPI). Experimental measurements using a Pockels voltage probe and gelling chemical reagent showed that electric field of 150 kV/cm and hydroxyl radical (OH) supply to cell cultures were induced by DPI under a typical condition compared with IPI. In addition, significant inhibition of the OH scavenger D‐mannitol indicated involvement of OH in those DPI‐induced cellular responses. These results facilitate the elucidation of plasma‐cell interaction and the development of plasma devices for medical treatments.