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Low temperature plasma induced apoptosis in CNE‐2Z cells through endoplasmic reticulum stress and mitochondrial dysfunction pathways
Author(s) -
Song Wencheng,
Wang Enjun,
Gao Ying,
Wu Qibing,
Rao Shenghong,
Wang Hongzhi,
Bao Lingzhi
Publication year - 2018
Publication title -
plasma processes and polymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 74
eISSN - 1612-8869
pISSN - 1612-8850
DOI - 10.1002/ppap.201600249
Subject(s) - endoplasmic reticulum , apoptosis , unfolded protein response , reactive oxygen species , microbiology and biotechnology , mitochondrion , viability assay , chemistry , downregulation and upregulation , cell culture , nasopharyngeal carcinoma , long term potentiation , biology , biochemistry , medicine , genetics , receptor , gene , radiation therapy
This study is designed to investigate the effect of low temperature plasma (LTP) on the apoptosis of CNE‐2Z cell (human nasopharyngeal carcinoma cell line) and the potential mechanisms. We find out that LTP provoked the generation of reactive oxygen species (ROS) and reactive nitrogen species (RNS) in CNE‐2Z cells and significantly inhibit the cell viability and induce cell apoptosis. Furthermore, the antioxidant N‐acetyl cysteine (NAC) almost completely blocked LTP‐induced apoptosis. LTP triggered mitochondrial and endoplasmic reticulum (ER) stress‐induced apoptotic pathways indicated by significant upregulation of CHOP, p53, and Bax‐to‐Bcl‐2 ratio. This work will provide the molecular theoretical basis of clinical application of LTP for the treatment of human nasopharyngeal carcinoma.