Premium
A combined low‐pressure hydrogen peroxide evaporation plus hydrogen plasma treatment method for sterilization − Part 2: An intercomparison study of different biological systems
Author(s) -
Lackmann JanWilm,
Fiebrandt Marcel,
Raguse Marina,
Kartaschew Konstantin,
Havenith Martina,
Bandow Julia E.,
Moeller Ralf,
Awakowicz Peter,
Stapelmann Katharina
Publication year - 2017
Publication title -
plasma processes and polymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 74
eISSN - 1612-8869
pISSN - 1612-8850
DOI - 10.1002/ppap.201600199
Subject(s) - hydrogen peroxide , sterilization (economics) , hydrogen , plasma , chemistry , raman spectroscopy , spore , response surface methodology , materials science , chemical engineering , chromatography , biochemistry , organic chemistry , microbiology and biotechnology , biology , physics , optics , quantum mechanics , monetary economics , economics , foreign exchange market , engineering , foreign exchange
Low‐pressure plasmas are a promising alternative to modern sterilization processes. As plasma is a surface process, multilayered stacks of spores are a crucial challenge to overcome. Here, a combined process of condensed hydrogen peroxide and hydrogen plasma is analyzed for its efficacy against various spore concentrations showing a clear increase in efficacy using a combined process compared to the two steps used separately. Besides spores, protein contaminations are a major issue in clinics and the combined process is investigated for protein removal efficiency using the well‐established BSA model. Furthermore, RNase A serves as a difficult‐to‐inactivate protein model to investigate protein inactivation efficiency. Finally, inactivation mechanisms of RNase A with a special focus on sulfur‐based modifications are investigated using Raman spectroscopy.