Premium
Contribution of hydrogen peroxide to non‐thermal atmospheric pressure plasma induced A549 lung cancer cell damage
Author(s) -
Ma Jie,
Zhang Hao,
Cheng Cheng,
Shen Jie,
Bao Lingzhi,
Han Wei
Publication year - 2017
Publication title -
plasma processes and polymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 74
eISSN - 1612-8869
pISSN - 1612-8850
DOI - 10.1002/ppap.201600162
Subject(s) - hydrogen peroxide , a549 cell , catalase , oxidative stress , programmed cell death , nonthermal plasma , cell damage , chemistry , reactive oxygen species , dna damage , apoptosis , cell , cancer cell , intracellular , biophysics , microbiology and biotechnology , cancer , plasma , biochemistry , biology , dna , genetics , physics , quantum mechanics
Non‐thermal plasma (NTP) can induce cancer cell death which is supposed to be mediated by oxidative stress. In this work, the contribution of hydrogen peroxide (H 2 O 2 ) in NTP induced lung cancer cell damage is quantitatively studied. Our results also show signification production of H 2 O 2 in plasma activated medium (PAM). NTP treatment induce critical raising of cell death, apoptosis, and DNA double‐strand breaks (DSBs) in A549 cell. NTP treatment also cause mitochondrial dysfunction and intracellular ROS production in A549 cells. Furthermore, catalase efficiently protect cells from NTP induced A549 cell damage. This work clarified the contribution of H 2 O 2 to plasma induced cancer cell damage.