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Cyclopropylamine plasma polymers for increased cell adhesion and growth
Author(s) -
Manakhov Anton,
Landová Marie,
Medalová Jiřina,
Michlíček Miroslav,
Polčák Josef,
Nečas David,
Zajíčková Lenka
Publication year - 2017
Publication title -
plasma processes and polymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 74
eISSN - 1612-8869
pISSN - 1612-8850
DOI - 10.1002/ppap.201600123
Subject(s) - polymer , adhesion , dissolution , materials science , chemistry , layer (electronics) , analytical chemistry (journal) , cell adhesion , deposition (geology) , composite material , chromatography , organic chemistry , paleontology , sediment , biology
Plasma polymers with NH x groups were deposited from cyclopropylamine in low pressure radio frequency (RF) discharges. The amount of NH x decreased with increasing average power P av , whereas the layer dissolution in water decreased and high P av led to a slight film swelling. The C2C12 cells demonstrated very high adhesion to the layers regardless of the deposition conditions. Although the surface chemistry of amine‐rich layers should enhance the cell proliferation, the WST‐1 assay, and immunocytochemistry revealed lower cell proliferation and viability on the layers exhibiting above 18% of relative thickness loss in water. Promising results were obtained on the layers with better water stability and bearing 7–10% of NH x .