Premium
Monte Carlo Poration Model of Cell Membranes for Application to Plasma Gene Transfection
Author(s) -
Zerrouki Amel,
Yousfi Mohammed,
Rhallabi Ahmed,
Motomura Hideki,
Jinno Masafumi
Publication year - 2016
Publication title -
plasma processes and polymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 74
eISSN - 1612-8869
pISSN - 1612-8850
DOI - 10.1002/ppap.201500144
Subject(s) - transfection , membrane , monte carlo method , particle (ecology) , plasma , biophysics , reflection (computer programming) , materials science , nanotechnology , chemistry , chemical physics , gene , physics , computer science , biochemistry , biology , ecology , statistics , mathematics , quantum mechanics , programming language
A Monte Carlo poration model is developed to simulate formation pores of few nanometer of width through cell multilayer membranes when impacted by air plasma species. Each species is assumed as a super‐particle involving a large particle number. It is assumed a simple membrane model superposing four layers of phospholipids and proteins. Each layer is composed by super‐sites subjected to plasma macro‐processes (recombination, reflection, activation, opening). Electrons play a main role on super‐site activations and openings but ions and radicals have also their own role on pore formation. Judicious choices probabilities data of macro‐processes lead to sizes of pores compatible with gene transfection. Present model, validated by comparisons with measured rates of transfected and surviving cells, is a tool for understanding the mechanisms of gene transfection.