Premium
Biodeactivation of Lipopolysaccharide Correlates with Surface‐Bound NO 3 After Cold Atmospheric Plasma Treatment
Author(s) -
Bartis Elliot A. J.,
Luan Pingshan,
Knoll Andrew J.,
Graves David B.,
Seog Joonil,
Oehrlein Gottlieb S.
Publication year - 2016
Publication title -
plasma processes and polymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 74
eISSN - 1612-8869
pISSN - 1612-8850
DOI - 10.1002/ppap.201500072
Subject(s) - atmospheric pressure plasma , biomolecule , lipopolysaccharide , plasma , atmospheric pressure , chemistry , polymer , surface modification , materials science , analytical chemistry (journal) , nanotechnology , chromatography , organic chemistry , physics , meteorology , biology , quantum mechanics , endocrinology
Cold atmospheric plasma (CAP) treatment of biological surfaces results in important changes of biological functions, but little knowledge on specific surface‐chemical changes is available. We measured surface‐bound NO 3 on polymer and biomolecular films after CAP treatment. An O 2 /N 2 ‐based surface microdischarge was used to deactivate lipopolysaccharide (LPS), an immune‐stimulating biomolecule found in Gram negative bacteria. The observed LPS biodeactivation was highest for low N 2 concentrations in O 2 , increased roughly linearly with surface NO 3 , and then saturated. NO 3 was also observed after treatment by a very different source: an atmospheric pressure plasma jet operating with an Ar carrier gas. Thus, NO 3 formation is a generic surface chemical modification of these materials by CAP sources.