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Atmospheric‐Pressure Plasma Deposited Epoxy‐Rich Thin Films as Platforms for Biomolecule Immobilization—Application for Anti‐Biofouling and Xenobiotic‐Degrading Surfaces
Author(s) -
Camporeale Giuseppe,
MorenoCouranjou Maryline,
Bonot Sébastien,
Mauchauffé Rodolphe,
Boscher Nicolas D.,
Bebrone Carine,
Van de Weerdt Cécile,
Cauchie HenryMichel,
Favia Pietro,
Choquet Patrick
Publication year - 2015
Publication title -
plasma processes and polymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 74
eISSN - 1612-8869
pISSN - 1612-8850
DOI - 10.1002/ppap.201400206
Subject(s) - biomolecule , surface modification , chemical engineering , materials science , atmospheric pressure plasma , biofouling , epoxy , aqueous solution , atmospheric pressure , laccase , biosensor , immobilized enzyme , plasma , deposition (geology) , nanotechnology , chemistry , organic chemistry , enzyme , composite material , paleontology , biochemistry , physics , oceanography , quantum mechanics , membrane , sediment , geology , engineering , biology
In this work, an atmospheric‐pressure dielectric barrier discharge process is exploited for the fast deposition of adherent epoxy‐rich layers acting as a versatile platform for the efficient one‐step biomolecule immobilization in mild aqueous conditions. Particular attention is given to the influence of the plasma process parameters on the chemical and morphological properties of the deposited layers and on their subsequent exploitation for chemical interfacial reactions. As a proof‐of‐concept, two enzymes with drastically different biological properties, namely dispersin B and a laccase, are immobilized onto functionalized metallic surfaces. The pH of the enzyme solution appears as a key parameter to control the amount of immobilized enzyme on the plasma functionalized surfaces, thus leading to bioactive surfaces with improved stability and activity.