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Dielectric Barrier Discharge Plasma Efficiently Delivers an Apoptotic Response in Human Monocytic Lymphoma
Author(s) -
Kaushik Neha,
Kumar Naresh,
Kim Chung Hyeok,
Kaushik Nagendra Kumar,
Choi Eun Ha
Publication year - 2014
Publication title -
plasma processes and polymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 74
eISSN - 1612-8869
pISSN - 1612-8850
DOI - 10.1002/ppap.201400102
Subject(s) - dielectric barrier discharge , apoptosis , reactive oxygen species , lymphoma , peripheral blood mononuclear cell , endogeny , plasma , u937 cell , chemistry , materials science , biophysics , microbiology and biotechnology , immunology , dielectric , biology , biochemistry , optoelectronics , in vitro , physics , quantum mechanics
In this paper, we demonstrate the effect of dielectric barrier discharge (DBD) plasma on lymphoma and normal blood mononuclear cells. Results indicates enhanced level of reactive oxygen species in U937 cells, which presented a compromised redox status evident from increased NADP + /NADPH levels, reduced GSH/GSSG ratios and decreases in the total endogenous antioxidant activity of cells. A plasma treatment drastically reduces cell viability, causing more severe damage to DNA and mitochondria, as determined by means of apoptosis indicators and related genes expression. Taken together, these findings indicate a significant level of apoptosis in plasma‐treated lymphoma cells. This report lends insight into progress toward an appropriate therapy for blood cancers using non‐thermal DBD plasma.