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Inactivation and Heme Degradation of Horseradish Peroxidase Induced by Discharge Plasma
Author(s) -
Ke Zhigang,
Huang Qing
Publication year - 2013
Publication title -
plasma processes and polymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 74
eISSN - 1612-8869
pISSN - 1612-8850
DOI - 10.1002/ppap.201300035
Subject(s) - horseradish peroxidase , heme , chemistry , hydrogen peroxide , peroxidase , photochemistry , radical , plasma , hemeprotein , reactive oxygen species , biophysics , biochemistry , enzyme , physics , quantum mechanics , biology
The effects of non‐thermal plasma at the argon gas–solution interface on horseradish peroxidase (HRP), a classical heme‐containing protein, were assessed quantitatively. Evidence from spectroscopic approaches showed that enzymatic activity together with the contents of heme and iron in HRP decreased dramatically and concomitantly upon plasma treatment. The plasma induced both long‐ and short‐lived reactive species which inactivated HRP in the solution. In particular, hydrogen peroxide broke the heme prosthetic group into fluorescent products, mainly dipyrrols and their derivatives, while other factors such as hydroxyl radicals and UV radiation generated during plasma discharge accelerated this heme protein degradation process.