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Thromboelastography ( TEG ) Cups and Pins with Different PECVD Coatings: Effect on the Coagulation Cascade in Platelet‐poor Blood Plasma
Author(s) -
ContrerasGarcía Angel,
Merhi Yahye,
Ruiz JuanCarlos,
Wertheimer Michael R.,
Hoemann Caroline D.
Publication year - 2013
Publication title -
plasma processes and polymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 74
eISSN - 1612-8869
pISSN - 1612-8850
DOI - 10.1002/ppap.201300027
Subject(s) - thromboelastography , chemistry , wetting , fibrin , plasma enhanced chemical vapor deposition , kinetics , clot formation , coagulation , chemical engineering , factor xii , biophysics , chromatography , organic chemistry , chemical vapor deposition , immunology , psychology , physics , quantum mechanics , psychiatry , biology , engineering
Thromboelastography uses cups and pins made of Cyrolite® plastic to analyze the rate of fibrin clot formation in blood samples. In this study, TEG cups and pins were modified by 4 distinct coating types using plasma‐enhanced chemical vapor deposition (PECVD): carboxylated, amine‐rich, hydrophobic, SiO 2 , and analyzed for surface chemistry and wettability. We tested the hypothesis that the coagulation kinetics of recalcified citrated blood plasma is controlled by surface chemistry, in the absence of clot activator. Only carboxylated surfaces became negatively charged upon wetting, and accelerated clot formation in a highly reproducible manner, whereas Cyrolite® and the other coatings had delayed and unpredictable clotting times. These data are consistent with a model whereby carboxylated surfaces selectively adsorb and activate factor XII while repelling other more abundant anionic blood proteins, resulting in reproducible clot kinetics.