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Formation of ROS and RNS in Water Electro‐ S prayed through Transient Spark Discharge in Air and their Bactericidal Effects
Author(s) -
Machala Zdenko,
Tarabova Barbora,
Hensel Karol,
Spetlikova Eva,
Sikurova Libusa,
Lukes Petr
Publication year - 2013
Publication title -
plasma processes and polymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 74
eISSN - 1612-8869
pISSN - 1612-8850
DOI - 10.1002/ppap.201200113
Subject(s) - hydrogen peroxide , ozone , chemistry , hydroxyl radical , dielectric barrier discharge , oxidative stress , spark discharge , hydrogen , reactive oxygen species , nonthermal plasma , plasma , bacteria , metal , oxidative phosphorylation , environmental chemistry , biophysics , biochemistry , radical , electrode , organic chemistry , physics , quantum mechanics , biology , genetics
Chemical and bactericidal effects induced by plasma in water upon electro‐spraying through DC‐driven positive transient spark discharge in air were investigated. Inactivation of E. coli was determined in dependence on pH (controlled by buffers) and correlated with chemical changes induced in water. Productions of hydrogen peroxide, nitrites, nitrates, peroxynitrites, and pH changes were determined, and the extent of oxidative stress induced in bacteria was evaluated. The degree of inactivation and oxidative damage of bacteria increased with the increasing acidity of the solution. Acidified nitrites interacting with hydrogen peroxide were determined as the most important bactericidal ROS/RNS agents in plasma‐treated water. A possible role of peroxynitrites, ozone, and metal nanoparticles is discussed.