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Antimicrobial Efficacy of an Atmospheric Pressure Plasma Jet Against Biofilms of Pseudomonas aeruginosa and Staphylococcus epidermidis
Author(s) -
Matthes Rutger,
Koban Ina,
Bender Claudia,
Masur Kai,
Kindel Eckhard,
Weltmann KlausDieter,
Kocher Thomas,
Kramer Axel,
Hübner NilsOlaf
Publication year - 2013
Publication title -
plasma processes and polymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 74
eISSN - 1612-8869
pISSN - 1612-8850
DOI - 10.1002/ppap.201100133
Subject(s) - biofilm , staphylococcus epidermidis , microbiology and biotechnology , antimicrobial , pseudomonas aeruginosa , antiseptic , chlorhexidine , chemistry , staphylococcus aureus , bacteria , medicine , biology , dentistry , organic chemistry , genetics
Microbial biofilms impair wound healing and the efficacy of antiseptics. This study used the plasma source kinpen 09 to investigate the efficacy of argon and an argon/oxygen‐mixture plasma against in vitro biofilms of Pseudomonas aeruginosa and Staphylococcus epidermidis compared to the antiseptic chlorhexidine digluconate (CHX). Biofilms were treated for 30, 60, 150, or 300 s with plasma and for 600 s with a 0.1% CHX solution. Thereafter, the biofilms were dispersed and their inactivation was determined as the reduction of colony forming units. Plasma treatment led to a significant antimicrobial effect compared to the untreated control and was at least as effective as the treatment with CHX, or even better, depending on the gas admixture, the bacterial strain used, and the exposure time.