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Derivatization of Plasma Polymerized Thin Films and Attachment of Biomolecules to Influence HUVEC‐Cell Adhesion
Author(s) -
Lotz Alexander,
Heller Martin,
Brieger Jürgen,
Gabriel Matthias,
Förch Renate
Publication year - 2012
Publication title -
plasma processes and polymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 74
eISSN - 1612-8869
pISSN - 1612-8850
DOI - 10.1002/ppap.201100121
Subject(s) - allylamine , adhesion , biomolecule , polymerization , covalent bond , polymer chemistry , linker , cell adhesion , surface modification , chemistry , umbilical vein , fibronectin , biophysics , derivatization , plasma polymerization , molecule , human umbilical vein endothelial cell , materials science , chemical engineering , biochemistry , chromatography , organic chemistry , cell , polymer , polyelectrolyte , biology , high performance liquid chromatography , computer science , engineering , in vitro , operating system
Plasma polymerized films of allylamine were surface derivatized in subsequent wet chemical processes using a diepoxy‐PEG linker to covalently bind different biomolecules that are known to influence cell adhesion. The initial attachment of human umbilical vein endothelial cells (HUVECs) was studied on (i) unmodified PTFE, (ii) PTFE modified with a layer of plasma polymerized allylamine (pp‐AA), and (iii) PTFE modified with a layer pp‐AA which was further modified using known adhesion molecules coupled to the functional surface via a linker molecule. Statistical analysis of the data initially show significant adhesion improvement of HUVECs on pp‐AA and on pp‐AA modified with either L‐RGD or C‐RGD in comparison to the bare PTFE. The statistical significances between the pp‐AA surface and the modified pp‐AA/RGD surface were however found to be negligible. A significant further improvement was observed for the modified surfaces derivatized with the very much larger fibronectin.