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The Role of Albumin and Fibronectin in the Adhesion of Fibroblasts to Plasma Polymer Surfaces
Author(s) -
Zelzer Mischa,
Albutt Darren,
Alexander Morgan R.,
Russell Noah A.
Publication year - 2012
Publication title -
plasma processes and polymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 74
eISSN - 1612-8869
pISSN - 1612-8850
DOI - 10.1002/ppap.201100054
Subject(s) - fibronectin , adhesion , protein adsorption , albumin , polymer , quartz crystal microbalance , adsorption , chemistry , biomaterial , blood proteins , cell adhesion , biophysics , allylamine , chemical engineering , polymer chemistry , surface modification , plasma polymerization , materials science , biochemistry , polymerization , organic chemistry , cell , polyelectrolyte , biology , engineering
Plasma polymer coatings are widely applied to the modification of biomaterial surfaces. In order to understand the role of the surface in the adhesion of cells to these materials, it is necessary to understand how protein adsorption is influenced by the plasma polymer surface chemistry. In coated scaffolds, plasma polymerised allylamine has been found to encourage fibroblast adhesion while plasma polymerised hexane reduces cell adhesion. To study the role of proteins in this process, albumin and fibronectin are pre‐adsorbed individually, competitively or sequentially to the plasma polymer surfaces before seeding them with a culture of 3T3 fibroblasts. Significant dependence upon the protein pre‐adsorption was seen in the adhered cell numbers. In situ measurements of protein adsorption using a quartz crystal microbalance help to elucidate the factors that govern the observed cellular response. The measured protein adsorption is rationalised in terms of our knowledge of the surface chemistry of these plasma polymers. We found that high cell adhesion is related to the ability of fibronectin to displace albumin. This ability is reduced on the hydrophobic plasma polymer as well as by adsorbing albumin and fibronectin sequentially.