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Improved Hemocompatibility Guided by Pulsed Plasma Tailoring the Surface Amino Functionalities of TiO 2 Coating for Covalent Immobilization of Heparin
Author(s) -
Yang Zhilu,
Wang Jin,
Luo Rifang,
Li Xin,
Chen Si,
Sun Hong,
Huang Nan
Publication year - 2011
Publication title -
plasma processes and polymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 74
eISSN - 1612-8869
pISSN - 1612-8850
DOI - 10.1002/ppap.201000101
Subject(s) - covalent bond , amine gas treating , adhesion , surface modification , coating , allylamine , chemical engineering , heparin , materials science , polymer chemistry , chemistry , polymer , organic chemistry , nanotechnology , biochemistry , polyelectrolyte , engineering
This work aims to develop a facile and effective approach to tailoring amine functional groups on the TiO 2 surface by depositing a thin film of plasma polymeric allylamine which is rich in amine groups. Furthermore, the primary amine groups on the surface are used to covalently immobilize heparin. The results reveal that heparin bound to the amine functionalized TiO 2 surface not only shows a good retention, but also retains biological activity. The hemocompatibility evaluation shows less platelet adhesion, platelet activation and fibrinogen activation on the heparin immobilized TiO 2 surface. Amine functionalized TiO 2 surface remarkably promotes endothelial cell (EC) adhesion and proliferation. On the heparin immobilized TiO 2 surface, although EC adhesion and proliferation is slightly suppressed at the beginning, after cultivation for 3 d, the growth behavior of ECs is obviously improved over pure TiO 2 surface.