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Inactivation of Staphylococcus aureus in Water by a Cold, He/O 2 Atmospheric Pressure Plasma Microjet
Author(s) -
Bai Na,
Sun Peng,
Zhou Haixia,
Wu Haiyan,
Wang Ruixue,
Liu Fuxiang,
Zhu Weidong,
Lopez Jose L.,
Zhang Jue,
Fang Jing
Publication year - 2011
Publication title -
plasma processes and polymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 74
eISSN - 1612-8869
pISSN - 1612-8850
DOI - 10.1002/ppap.201000078
Subject(s) - staphylococcus aureus , distilled water , atmospheric pressure , oxygen , spectroscopy , plasma , chemistry , analytical chemistry (journal) , atmospheric pressure plasma , helium , fluorescence , fluorescence spectroscopy , bacteria , environmental chemistry , chromatography , optics , biology , organic chemistry , genetics , oceanography , physics , quantum mechanics , geology
A direct‐current, atmospheric pressure, cold plasma microjet (PMJ) sustained in a quasi‐steady gas cavity in liquid was used to inactivate Staphylococcus aureus suspended in distilled water. While helium gas (with 2% O 2 as additive) was used as working gas, an effective inactivation (>99%) was achieved in 6 min. The inactivation of bacteria was further verified by surface morphology examination and LIVE/DEAD Baclight bacterial viability test (fluorescence microscopy). The overall pH and temperature of the liquid were monitored during the plasma treatment and were found to be below the critical values for the survival of S. aureus. Hydroxyl radical ( • OH) was detected via electron spin resonance (ESR) spectroscopy, and alongside other intermediate reactive species, is attributed to the effective inactivation of S. aureus . End‐on optical emission spectroscopy show strong atomic oxygen emission both in air and in water.