Premium
Biological Evaluation of DNA Damage in Bacteriophages Inactivated by Atmospheric Pressure Cold Plasma
Author(s) -
Yasuda Hachiro,
Miura Takuya,
Kurita Hirofumi,
Takashima Kazunori,
Mizuno Akira
Publication year - 2010
Publication title -
plasma processes and polymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 74
eISSN - 1612-8869
pISSN - 1612-8850
DOI - 10.1002/ppap.200900088
Subject(s) - atmospheric pressure plasma , dna , atmospheric pressure , dna damage , plasma , nonthermal plasma , in vitro , in vivo , chemistry , microorganism , biophysics , microbiology and biotechnology , bacteria , materials science , biology , biochemistry , genetics , physics , quantum mechanics , meteorology
Inactivation mechanisms of plasma treated microorganisms are still major subjects. We have developed a biological assay which evaluates in vivo DNA damage of the viruses treated with non‐thermal atmospheric pressure plasma in air. Different doses of the plasma were applied to wet state of λ phage particles under neutral pH and near the room temperature. From each sample of treated λ phages, DNA was purified and subjected to in vitro DNA packaging reactions. Survival curves of the re‐packaged phages showed an extremely large D value ( D = 25 s) compared to the previous D value ( D = 3 s) obtained from the survival curves of the treated phages. The results indicate the evidence that DNA damage hardly contributed to the inactivation.