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Influence of Cold Plasma Atmospheric Jet on Surface Integrin Expression of Living Cells
Author(s) -
Shashurin Alexey,
Stepp Mary Ann,
Hawley Teresa S.,
PalGhosh Sonali,
Brieda Lubos,
Bronnikov Svetlana,
Jurjus Rosalyn A.,
Keidar Michael
Publication year - 2010
Publication title -
plasma processes and polymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 74
eISSN - 1612-8869
pISSN - 1612-8850
DOI - 10.1002/ppap.200900086
Subject(s) - integrin , flow cytometry , cell , microbiology and biotechnology , atmospheric pressure plasma , chemistry , fluorescence microscope , biophysics , materials science , fluorescence , plasma , biology , biochemistry , optics , physics , quantum mechanics
The effects induced in cells due to treatment with cold atmospheric plasma jet are studied. Cell migration rate is measured by means of time‐lapse microscopy. In order to characterize cell surface integrin expression, the fluorescent response of cells after surface integrins are stained with specific antibodies is measured by flow cytometry. We show that treatment of cells with plasma jet affects the cells on sub‐cellular level, namely decreases expression of cell surface integrins ( β 1 and α v integrins were tested). This change in integrin expression might be the original cause for the effects observed on cellular level, such as reduced cell migration rate and cell detachment observed experimentally.