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Antibacterial Activity of an Atmospheric Pressure Plasma Jet Against Relevant Wound Pathogens in vitro on a Simulated Wound Environment
Author(s) -
Daeschlein Georg,
von Woedtke Thomas,
Kindel Eckhard,
Brandenburg Ronny,
Weltmann KlausDieter,
Jünger Michael
Publication year - 2010
Publication title -
plasma processes and polymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 74
eISSN - 1612-8869
pISSN - 1612-8850
DOI - 10.1002/ppap.200900059
Subject(s) - microbiology and biotechnology , staphylococcus aureus , pseudomonas aeruginosa , agar plate , in vitro , candida albicans , enterococcus faecium , agar , atmospheric pressure plasma , bacteria , chemistry , biology , antibiotics , plasma , biochemistry , genetics , physics , quantum mechanics
The aim of the study was to test the efficacy of a hand‐held atmospheric pressure plasma jet (APPJ) toward typical wound pathogens in vitro simulating antisepsis on wound surfaces. The plasma jet has been proved to be highly effective in vitro against the most commonly encountered pathogenic species of acute and chronic wounds reaching nearly the power of antiseptics. The following bacteria and fungi were treated on half rigid media (agar) imitating wound colonization: methicillin‐sensitive Staphylococcus aureus ATCC 1924 (MSSA), Enterococcus faecium ATCC 6057 (EF), Pseudomonas aeruginosa ATCC 15442 (PA), Candida albicans ATCC 10231 (CA), and β ‐hemolyzing Streptococci of the Lancefield serogroup A (HSA). Highest reduction factor (RF) was obtained treating PA (RF 4.0) followed by HSA (3.2), MSSA (2.7), CA (2.0), and EF (1.9). Consequently, simulating wound surfaces with moist environment using semisolid agar media, the APPJ allowed bactericidal treatment of highly contaminated surfaces of 55 cm 2 imitating skin and wound colonization within 6 min. This antibacterial reduction power together with its handsome flexibility of the APPJ could be a suited therapeutic option in the therapy of infected or colonized wounds.