Premium
States of Biological Components in Bacteria and Bacteriophages during Inactivation by Atmospheric Dielectric Barrier Discharges
Author(s) -
Yasuda Hachiro,
Hashimoto Mai,
Rahman Md. Masudur,
Takashima Kazunori,
Mizuno Akira
Publication year - 2008
Publication title -
plasma processes and polymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 74
eISSN - 1612-8869
pISSN - 1612-8850
DOI - 10.1002/ppap.200800036
Subject(s) - bacteriophage , escherichia coli , dielectric barrier discharge , sterilization (economics) , bacteria , denaturation (fissile materials) , chemistry , biophysics , degradation (telecommunications) , peptide , dna , microbiology and biotechnology , biochemistry , dielectric , biology , materials science , nuclear chemistry , gene , genetics , telecommunications , computer science , monetary economics , economics , foreign exchange market , foreign exchange , optoelectronics
Atmospheric DBD has been applied to the wet state of Escherichia coli and bacteriophage‐ λ . Upon DBD treatment, both E.coli and λ phage were immediately inactivated. The states of different biological components were monitored during the course of inactivation. Only minor and slow degradation of proteins, DNA, and membranes was observed, a remarkable degradation was seen only after the completion of sterilization. Analysis of GFP recombinantly introduced into E.coli cells proved that the DBD has a prominent protein denaturation activity without affecting peptide bonds. The irreversible denaturation of proteins, seen in the early stage of DBD application, may play a central role in inactivation of both the bacteria and bacteriophages.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom