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States of Biological Components in Bacteria and Bacteriophages during Inactivation by Atmospheric Dielectric Barrier Discharges
Author(s) -
Yasuda Hachiro,
Hashimoto Mai,
Rahman Md. Masudur,
Takashima Kazunori,
Mizuno Akira
Publication year - 2008
Publication title -
plasma processes and polymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 74
eISSN - 1612-8869
pISSN - 1612-8850
DOI - 10.1002/ppap.200800036
Subject(s) - bacteriophage , escherichia coli , dielectric barrier discharge , sterilization (economics) , bacteria , denaturation (fissile materials) , chemistry , biophysics , degradation (telecommunications) , peptide , dna , microbiology and biotechnology , biochemistry , dielectric , biology , materials science , nuclear chemistry , gene , genetics , telecommunications , computer science , monetary economics , economics , foreign exchange market , foreign exchange , optoelectronics
Atmospheric DBD has been applied to the wet state of Escherichia coli and bacteriophage‐ λ . Upon DBD treatment, both E.coli and λ phage were immediately inactivated. The states of different biological components were monitored during the course of inactivation. Only minor and slow degradation of proteins, DNA, and membranes was observed, a remarkable degradation was seen only after the completion of sterilization. Analysis of GFP recombinantly introduced into E.coli cells proved that the DBD has a prominent protein denaturation activity without affecting peptide bonds. The irreversible denaturation of proteins, seen in the early stage of DBD application, may play a central role in inactivation of both the bacteria and bacteriophages.