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Delayed Effects of Cold Atmospheric Plasma on Vascular Cells
Author(s) -
Stoffels Eva,
Roks Anton J. M.,
Deelman Leo E.
Publication year - 2008
Publication title -
plasma processes and polymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 74
eISSN - 1612-8869
pISSN - 1612-8850
DOI - 10.1002/ppap.200800028
Subject(s) - necrosis , cell , atmospheric pressure plasma , biophysics , apoptosis , endothelial stem cell , plasma , chemistry , membrane , cell membrane , microbiology and biotechnology , materials science , pathology , medicine , biology , biochemistry , in vitro , physics , quantum mechanics
Abstract We investigated the long‐term behaviour of vascular cells (endothelial and smooth muscle) after exposure to a cold atmospheric plasma source. The cells were treated through a gas‐permeable membrane, in order to simulate intravenous treatment with a gas plasma‐filled catheter. Such indirect treatment resulted in (dose‐dependent) apoptosis and necrosis in smooth muscle cells; these effects were visible 6–10 h after exposure. We ascribed cell damage to poisoning by active nitrogen species. A not yet resolved phenomenon of total (irreversible) cell inactivation without necrosis was observed in endothelial cells. Conditions for non‐inflammatory plasma treatment (no cell necrosis) can be realized. Furthermore, the current experiments have brought new insights in the mechanism of cell detachment, which was observed in many previous studies.