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Controlled Cell Attachment, Using Plasma Deposited Polymer Microstructures: A Novel Study of Cells‐Substrate Interactions
Author(s) -
Sciarratta Vincenzo,
Sohn Kai,
BurgerKentischer Anke,
Brunner Herwig,
Oehr Christian
Publication year - 2006
Publication title -
plasma processes and polymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 74
eISSN - 1612-8869
pISSN - 1612-8850
DOI - 10.1002/ppap.200600026
Subject(s) - biocompatibility , substrate (aquarium) , materials science , plasma polymerization , adhesion , microstructure , cell adhesion , polymer substrate , polymer , adsorption , biophysics , polymerization , nanotechnology , protein adsorption , chemical engineering , chemistry , composite material , organic chemistry , oceanography , engineering , metallurgy , biology , geology
Summary: Several actual developments in medical therapy are focussing on the potential of a surface‐dependent selection and proliferation of special cell types. For an improvement of biocompatibility it is interesting to understand the interactions between cells and material surfaces in order to create devices with the respective characteristics. In this paper the possibility to isolate three different tumor cells lines are studied: HEK293, RINm5f and KYM‐1. A procedure by means of plasma polymerization is demonstrated to create hydrophilic microstructures (precursor: AAc) on a hydrophobic (precursor: CHF 3 ) substrate and shown to effectively select RINm5f cells. Moreover, by studying the interaction between culture media and deposited polymers, it is shown that Ca 2+ ions and protein adsorption play a fundamental role in the cell‐substrate adhesion and proliferation.